Thursday, 27 September 2012 at 3:37 pm

Correlations are commonly calculated in RNA-seq data to check whether there are any dependence between two sets of data. For example technical or biological replicates are expected to be closely correlated, ie. similar expression values for transcripts between two samples. For an more in depth explanation of correlation, check out my previous post.

Pearson and Spearman are two widely used methods for calculating correlation. In this post, I will attempt to explain the differences between the two methods and their usage in RNA-seq data.